Affiliation:
1. Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey Piscataway New Jersey USA
2. Environmental and Occupational Health Sciences Institute Rutgers, The State University of New Jersey Piscataway New Jersey USA
3. Department of Biostatistics and Epidemiology, School of Public Health Rutgers, The State University of New Jersey Piscataway New Jersey USA
4. Department of Environmental and Occupational Health and Justice Rutgers, The State University of New Jersey Piscataway New Jersey USA
5. Research and Early Development Nonclinical Safety, Bristol Myers Squibb New Brunswick New Jersey USA
Abstract
AbstractChronic disruption of circadian rhythms by night shift work is associated with an increased breast cancer risk. However, little is known about the impact of night shift on peripheral circadian genes (CGs) and circadian‐controlled genes (CCGs) associated with breast cancer. Hence, we assessed central clock markers (melatonin and cortisol) in plasma, and peripheral CGs (PER1, PER2, PER3, and BMAL1) and CCGs (ESR1 and ESR2) in peripheral blood mononuclear cells (PBMCs). In day shift nurses (n = 12), 24‐h rhythms of cortisol and melatonin were aligned with day shift‐oriented light/dark schedules. The mRNA expression of PER2, PER3, BMAL1, and ESR2 showed 24‐h rhythms with peak values in the morning. In contrast, night shift nurses (n = 10) lost 24‐h rhythmicity of cortisol with a suppressed morning surge but retained normal rhythmic patterns of melatonin, leading to misalignment between cortisol and melatonin. Moreover, night shift nurses showed disruption of rhythmic expressions of PER2, PER3, BMAL1, and ESR2 genes, resulting in an impaired inverse correlation between PER2 and BMAL1 compared to day shift nurses. The observed trends of disrupted circadian markers were recapitulated in additional day (n = 20) and night (n = 19) shift nurses by measurement at early night and midnight time points. Taken together, this study demonstrated the misalignment of cortisol and melatonin, associated disruption of PER2 and ESR2 circadian expressions, and internal misalignment in peripheral circadian network in night shift nurses. Morning plasma cortisol and PER2, BMAL1, and ESR2 expressions in PBMCs may therefore be useful biomarkers of circadian disruption in shift workers.
Funder
National Institute of Environmental Health Sciences
Cited by
1 articles.
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1. Hypertension disrupts the vascular clock in both sexes;American Journal of Physiology-Heart and Circulatory Physiology;2024-10-01