Metrological Characteristics of the Potentiometric Assay Developed for Determining the Antioxidant Activity of Ascorbic Acid

Author:

Maslov Oleksandr Yu.1ORCID,Kolisnyk Sergii V.1ORCID,Komisarenko Mykola A.1ORCID,Akhmedov Elshan Yu.1ORCID,Koval Alla O.1ORCID,Kostina Tatiana A.1ORCID,Kolisnyk Olena V.1ORCID

Affiliation:

1. National University of Pharmacy of the Ministry of Health of Ukraine, Ukraine

Abstract

The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2 = 0.9993). The percentage of recovery was found to be in the range from 95.38 to 105.00 %. The values of %RSD for repeatability and intermediate precision were 1.86 and 1.95 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %. It has been proven that the method developed is express, rapid, highly sensitive, accurate and sufficiently reliable.

Publisher

National University of Pharmacy

Subject

General Medicine

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