Impact of Gibberellic Acid and Naphthalene Acetic Acid on Axillary Shoot Multiplication in Hygrophila polysperma (Roxb.) T. Anderson

Abstract

In this work, axillary shoot regenerations were investigated from nodal explants of Hygrophila polysperma (Roxb.) T. Anderson in MS medium (Murashige and Skoog) including different combinations of Gibberellic Acid (GA3) and Naphthalene Acetic Acid (NAA). Nodal explants of H. polysperma were used in propagation studies. The 100% regeneration frequency was determined in MS plus 0.25 and 0.50 mg/L GA3 + 0.15 mg/L NAA. When the shoot numbers were examined, the best nutrient medium was determined as 0.25 mg/L GA3 + 0.15 mg/L NAA (14.72 shoots/explant). The best result in shoot length was reached in MS plus 0.50 mg/L GA3 + 0.15 mg/L NAA (1.91 cm). Then the longest shoot was determined as 1.79 cm in nutrient media plus 0.25 mg/L GA3 + 0.15 mg/L NAA. Longer shoots were obtained in GA3+NAA nutrient media according to control. When the results were examined, it was determined that the number and length of shoots decreased as the amount of GA3+NAA used in the culture medium increased. Regenerated shoots were rooted in MS media including 0.25 mg/L Indole-3-Acetic Acid and successfully acclimatized to aquarium conditions. As a result, multiple and rapid productions of H. polysperma under tissue culture conditions were achieved.