Characteristics of neutrophilic granulocytes of peripheral blood in patients with mechanical jaundice caused by cholangiocarcinoma-Reference-Cited by-同舟云学术

Characteristics of neutrophilic granulocytes of peripheral blood in patients with mechanical jaundice caused by cholangiocarcinoma

Published:2022-03-10 Issue:1 Volume:24 Page:89-96
ISSN:2313-741X
Container-title:Medical Immunology (Russia)
language:
Short-container-title:Med. immunol.

Author:

Smirnova O. V.¹,Gubanov B. G.¹,Kasparov E. V.¹,Darenskaya M. A.²,Kolesnikova L. I.²,Kolesnikov S. I.²

Affiliation:

1. Research Institute of Medical Problems of the North, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences

2. Research Centre for Family Health and Human Reproduction Problems

Abstract

Obstructive jaundice caused by cholangiocarcinoma takes a special place among malignant disorders and surgical pathology in Russia. Involvement of nonspecific immunity and the role of neutrophils in carcinogenesis are ambiguously evaluated. The aim of this study was to study functional activity of peripheral blood neutrophilic granulocytes and their phenotype in obstructive jaundice caused by cholangiocarcinoma. The study included 56 patients with obstructive jaundice associated with cholangiocarcinoma at the T2- 3N0- 1M0 stage (clinical stages II-III), and 90 apparently healthy volunteers of similar age group. Neutrophilic granulocytes were isolated from peripheral blood by means of double-density Ficoll-Urografin gradient.Venous blood was collected in patients into vacutainers with heparin upon admission to the hospital, before the surgery was performed. Spontaneous cytokine production (IL-8, IFNá) was determined by enzyme-linked immunosorbent assay using Vector-Best diagnostic kits. To assess phagocytic activity of neutrophilic granulocytes, the phagocytic index (according to Hamburger), number of phagocytes (according to Wright) and the index of completed phagocytosis (according to Rudik, 2006) were calculated. Immunophenotyping of neutrophilic granulocytes was carried out using an FC500 flow cytometer (Beckman Coulter, USA) with monoclonal antibodies to CD11b+, CD16+, CD95+. The results were statistically analyzed using the Statistica v.12.0 software (StatSoft Inc., USA). To assess intergroup differences, the nonparametric Kruskal-Wallis tests (for three or more comparison groups) and Mann-Whitney tests (for pairwise comparison) were used. Comparison of groups for a qualitative binary trait was carried out using the two-sided Fisher’s exact test. Data are presented as Median (25 quartile-75 quartile).The study of the functional activity of peripheral blood neutrophiles in obstructive jaundice patients caused by cholangiocarcinoma revealed an increase in their relative and absolute number, increased phagocytic index and decreased phagocytic number of neutrophilic granulocytes, increased expression of CD11b+, CD16+, CD95+ immunological markers. The changes in neutrophil secretory activity were characterized by a decrease in cytokine production (IL-2, IFNá). An increase in functional activity of neutrophilic granulocytes, along with a decrease in their cytokine production suggests that, in obstructive jaundice observed in cholangiocarcinoma at clinical stage T2-3N0-1M0, an equilibrium stage is revealed between the cells of immune system and malignant tumor.

Publisher

SPb RAACI

Subject

Immunology,Immunology and Allergy

Reference31 articles.

1. Antoneeva I.I., Gening T.P. Neutrophilic granulocytes in the dynamics of ovarian cancer progression. Klinicheskaya laboratornaya diagnostika = Russian Clinical Laboratory Diagnostics, 2007, no. 8, pp. 43-45. (In Russ.)

2. Galperin E.I., Akhaladze G.G., Vetshev P.S., Dyuzheva T.G. Diﬀerential approach to the use of minimally invasive methods of treating neoplastic obstructive jaundice. Annaly khirurgicheskoy gepatologii = Annals of Surgical Hepatology, 2019, Vol. 24, no. 8, pp. 10-24. (In Russ.)

3. Dolgushin I.I, Shishkova Yu.S., Semenova A.B., Kazachkov E.L., Vazhenin A.V., Shamanova A.Yu., Dimov G.P. A look at the role of neutrophilic extracellular DNA as a component of the tumor microenvironment in the processes of carcinogenesis. Uralskiy meditsinskiy zhurnal = Ural Medical Journal, 2014, no. 2 (116), pp. 19-22. (In Russ.)

4. Zemskov A.M., Zemskova V.M., Zemskov M.A., Vorontsova Z.A., Zoloedov V.I. Innovative and analytical technologies based on the results traditional immunological monitoring of patients. Vestnik novykh meditsinskikh tekhnologiy = Bulletin of New Medical Technologies, 2019, Vol. 26, no. 2, pp. 40-43. (In Russ.)

5. Kotovsky A.E., Glebov K.G., Dyuzheva T.G., Syumareva T.A., Magomedova B.M. Retrograde bile duct endoprosthetics in benign diseases of the hepatopancreatoduodenal zone. Annaly khirurgicheskoy gepatologii = Annals of Surgical Hepatology, 2019, Vol. 24, no. 1, pp. 61-70. (In Russ.)