Improved micropropagation and salinity tolerance of strawberry (Fragaria X ananssa L) cv. Albion

Abstract

Gamma-ray has been used to increase genetic variation to obtain salt-tolerant plants in strawberries.The protocol was established to multiply strawberry cv. Albion from runner segments cultured on multiplication Murashige and Skoog (MS) medium contain 0.5 mg l-1 of 6-benzyl adenine (BA) and 0.1 mg l-1 of Naphthaleneacetic acid (NAA). Cultures were irradiated with gamma rays at (0, 20, 50, 100) Gy after 30 days, and the irradiated and unirradiated shoots were exposed to different concentrations of Sodium Chloride (NaCl) (6,10,14, 22) dS m-1. The results showed the superiority of doses 20 and 50 Gy in giving the highest rate of the number of shoots reached (9.25 and 8.44) shoot explant-1. The treatment 6 dS m-1 NaCl with 20 Gy was superior in giving the highest fresh 4.75 g and dry weight 0.36 g. A significant increase of proline was observed in the shoots irradiated with a dose of 50 Gy and grown on a medium with 22 mg l-1 of NaCl, as it reached 34.36 (‎µm‎ proline g-1 fresh weight) compared 6 dS m-1 and unirradiated media and the highest enzyme activity of (POD) was )263.50 units g-1 FW ( when treated with 100 Gy grown on a medium with 22 ds m-1 of salt. While the dose exceeded 20 Gy without adding salt, as it gave the highest activity of (CAT) enzyme, reaching )4.042 units g-1 FW(. It was observed that multiplication was generally restricted, depending on the increase in salt applications and gamma rays. Keywords: BA, NAA, Fragaria, Micropropagation, mutation gamma ray. Salt tolerance.