Synergistic Treatment of Candida albicans Infected Mice by AgNPs Synthesis Penicillium chrysogenum

Abstract

Penicillium chrysogenum has been used for the production of metal nanoparticles. It was experimentally shown that silver nitrate ions are reduced to silver nanoparticles in biomass, and silver nanoparticles are synthesized from 1 mM silver nitrate. The external morphology of ground AgNPs was established by SEM analysis and UV-visible spectrophotometry, and the size of AgNO3 particles was observed to be 23 nm, with particles being spherical by AFM. In vitro investigations, the antifungal susceptibility of Candida al was calculated, and the results showed that the inhibitory effect of AgNPs synthesized with P. chrysogenum is increased with increasing the concentration of nanoparticles, and the effective fungicidal concentration (EC50) was 1 ppm, and the minimum fungicidal concentration (MFC) was 100 ppm. Furthermore, the pathogenicity of C. albicans on kidneys in mice during infections was tested and thus indicated that the kidneys' ability was significantly enhanced when animals were treated with the combination of AgNPs and P. chrysogenum. This study's data from examination provide valuable preliminary statistics for using biosynthesized silver nanoparticles to manage various microorganisms. Keywords: Candida albicans, AgNPs Synthesis, Penicillium chrysogenum