Affiliation:
1. High Diploma, Institute of Genetic Engineering and Biotechnology, University of Baghdad
2. University of Baghdad, Institute of Genetic Engineering and Biotechnology
Abstract
Infertility is a disease that affects reproduction and is one of the most common
marital problems, which is considered a stigma in our society. It is considered a big
problem in countries, states and developing countries (third world countries) because of the modern lifestyle. This type of study is looking for the causes of this
problem. This study was conducted from January to June 20 in the Infertility Unit
in Baghdad, Iraq, on 100 men; 60 of the women were infertile, and 40 were healthy
and not infertile. Polymerase chain reaction and restriction fragment length polymorphism analyses were used for genotyping the rs 4938723 and rs28362491
SNPs.This study aims to study the genes NF-KB1 and Mir34b/c, to measure the
antibodies to sperm, and to know the immune role of interleukin-6 in infertile and
non-sterile men. The genes appeared with desirable and natural results. Where the
proportion of genes was (P≤0.05), these results were the lowest compared to previous studies on the same genes.
Keywords: DNA, Genotype, PCR, Blood,
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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