Staphylococcus aureus enterotoxin coding genes identified from patients with atopic dermatitis in Iraq by molecular analysis

Abstract

The present study determined the frequency of Staphylococcus aureus enterotoxins (B, C, Luk-pv) of atopic dermatitis isolated from (AD) cases in Laboratories of Baghdad. 54 Staphylococcus aureus isolates were subjected to primary identification tests using various methods (cultural characteristics, gram staining, biochemical tests, and vitek2 system). This study used antibiotic disc diffusion in fifty-four S. aureus isolates. Multidrug resistance (MDR) against different antimicrobial agents applied to polymerase chain reaction to amplify different genes coding for Staphylococcal enterotoxins, including 3 types (seb, sec and luk-pv), To ensure that the sequences of these genes match NCBI, DNA sequencing was performed for isolate No. (3). As a result of this study, 25 isolates had a multidrug resistance (MDR) percent (46.2%) against different antimicrobial agents—the results of DNA extraction and polymerase chain reaction directed to amplify the specific enterotoxin coding genes. This study showed that the (seb) gene is present in isolates of staph aureus bacteria isolated from patients with atopic dermatitis 12 /25 at a percentage (48 %). Furthermore, the absence of the sec gene in all Staphylococcus aureus isolates isolated from patients with atopic dermatitis—the results of the detection of the luk-pv 23\25 (92%) gene encoded for lukucidin.Polymerase chain reaction using different primers successfully identified Staphylococcus aureus enterotoxins (B, C)and luk-pv, luk-pv gene, which was the most frequent. Keywords: Staphylococcus aureus enterotoxin genes, polymerase chain reaction, Staphylococcus aureus, atopic dermatitis