Affiliation:
1. Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Abstract
This study was based on the importance of the effect of L. paracasei against E.coli
O157:H7 that was isolated from sheep suffering from diarrhea in many areas of
Baghdad (Abu-Ghraib, AL-Mahmoudia and AL-Yosifiya). All samples were cultivated on MacConkey agar, Eosine Methylene Blue and Sorbitol MacConkey agar
for E. coli isolation and then identified by biochemical tests. Out of 101 diarrhea
samples, 100 isolates gave positive E. coli results. The isolates of L. paracasei
were taken and cultured on conditions at 37ºC for 24 hours in Man Rogosa Sharpe
broth and incubated under CO2 (5-10%) for 24 hours, then recultured on MRS
agar, examined by gram stain and then confirmed diagnosis by Vitek2. Lactobacillus paracasei was examined against E. coli O157:H7 by well diffusion method
and measured the diameters of the inhibition zone around colonies. Mice (white
Balb) were used as laboratory animal models to investigate the effect and efficacy
of L. paracasei in treating diarrhea caused by E. coli O157; 50 mice were divided
into five groups. The histopathological examination of the intestine noticed
changes during infection with E.coli O157:H7 treated with probiotics.
Keywords: Vitek2; Laboratory technique; MacConkey agar; Histopathology;
Iraq.
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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