Seminal Fluid parameters abnormality in a sample of Iraqi Infertile Males with observation of the chromosomal abnormalities in an Azoospermia case by using Karyotyping

Author:

Mohammed Hamza Heba1,Al- Rubaye Dalal S.1,Ahmed Al-Khafaji Qays2,Almukhtar Asma A.3,M.Ali Amal3

Affiliation:

1. Department of Biotechnology, College of Science, Baghdad University, Baghdad, Iraq

2. Consultant Immunologist, Iraqi ministry of health

3. Iraqi Center for Cancer and medical genetics Research/ University of AL-Mustansiriya

Abstract

Male infertility is any health issue in a man that lowers the chances of his female partner getting pregnant. The present study aimed to observe the seminal fluid parameters abnormalities among primary and secondary infertility. The total number of participants is 93, consisting of 83 infertile males and 10 normal males as a control group. The patient's group was classified into Azoospermia, Asthenospermia and Oligoasthenospermia according to their semen abnormalities. The total number of patients is divided into two groups, the primary (65), who have no children and those with secondary infertility (18), who are unable to conceive or carry a baby to full-term after having a previous successful pregnancy. According to the seminal analysis, there were 18 infertile males with Azoospermia, 26 infertile males with oligoasthenospermia, 39 infertile males with asthenoospermia and 10 as a control group as represented. A one-way ANOVA is used to determine whether or not there is a statistically significant difference between the means of the analyzed independent groups. The mean of all parameters represents significant differences between the control group and other tested groups with few exceptions, e.g., the seminal volume in the control group showed no higher difference than Asthenospermia. Banding technique: G-band showing 46XY, der 2, der 3, der 4, der 7. And in der 5 but not in all cell.s Abnormal karyotype for males with a derivative of one copy of chromosome 2, one copy of chromosome 3 was a derivative, a copy of chromosome 4 was a derivative and a derivative of chromosome 7. Keywords: infertility 1; karyotype 2; Azoospermia 3; Asthenospermia 4; Oligoasthenospermia 5

Publisher

Clinical Biotec

Subject

Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology

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