A comparative study of the effect of nanoparticles and antibiotics on the antimicrobial susceptibility of antibiotics for some bacteria isolated from wounds and burns infections.

Abstract

This study aimed to investigate the isolation and identification of potential bacteria present in burns and wound infections. 100 pus types were taken from many patients who existed in the hospital of Babylon province from November 2021 to February 2022; the positive culture was 80% of the total isolates, while the negative culture was 20% of the total isolates. After the macroscopic, microscopic and biochemical tests, the bacteria were isolated from burn and wound swabs. Antibiotic susceptibility testing of the isolates was done using the disc diffusion method in conformity with McFarland standards. MDR of different types of antibiotics was exhibited from bacteria isolated in actual research. Statistical analysis using chisquare test The central objective of this research is to use different concentrations of Zinc oxide nanoparticles to obtain the optimum antibacterial concentration. Males (37.5%) and females (62.5%), with ages ranging from 5 to 55 years. The high percentage was less than ten years old, while the lowest was between (and 50_60). Where Gram-negative bacteria were most common, the concentration of Zinc oxide nanoparticles was 100 μg/ml. 150 μg/ml affects gram-positive and gram-negative growth by measuring the diameter of the inhibition zone on the growth culture that is artificial to Gram-positive and Gram-negative bacteria throughout the measuring inhibition zone around the wells. We observed that the diameter of the inhibition zone increased in concentration (150) μg/ml more than the concentration (100) μg/ml. The study showed women of positive growth and the ages compared to males. Multi-Drug Resistance bacteria. Zinc oxide nanoparticles have critical applications as they are antibacterial and effectively dress for burns and wounds . Keywords: Gram-positive Bacteria, Gram-negative bacteria, Wounds, Burns, Antimicrobial sensitivity, Multi-Drug Resistant, nanoparticles.