Affiliation:
1. Department of Horticulture and Landscape, Faculty of Agriculture, University of Kufa, Najaf, Iraq
Abstract
The study was carried out in the tissue culture laboratories at the College of Agriculture / University of Kufa during the period from April 2020 to October 2021
where the aim was to assess the efficiency of micropropagation of strawberries
by adding different concentrations of sucrose (30, 40 and 50 g.L-1
) to the nutrient
medium in combination with different cultivation systems: Semi-solid media system and temporary immersion system using the Plantform bioreactor. The results
showed that both the highest concentration of sucrose and the culture system under the medium immersion, individually or combined, were the highest and significantly effective in increasing the rate of number of shoots (runners), leaf content of macro and minor elements, total soluble sugars, plant pigments and fresh
weight of biomass. Generally, the highest values were recorded in the shoots
grown in liquid media supplied with 50 g.L-1
sucrose under temporary immersion
in the PlantForm bioreactor compared with those grown in semi-solid media.
Keywords: Fragaria × ananassa, micro-propagation, plant nutrition, tissue culture, PlantForm bioreactor
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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