Affiliation:
1. Department of Biology, College of Science, Mustansiriyah University Baghdad, Iraq
Abstract
L-asparagine is an enzyme widely identified as one of the most potential anticancerous drugs. This enzyme is poorly bioavailable, so its pharmaceutical uses
are limited as an anticancer. L-asparaginase is an important chemotherapeutic
drug used to treat a variety of malignant tumors. Lactic acid bacteria may exhibit
high L-asparaginase productivity, efficiency, and anticancer activity against breast
cancer cell lines. The L-asparaginase was extracted and purified in multiple
processes, including precipitation with (NH4)2SO4 (70%), DEAE-cellulose ion
exchanger chromatography, and Sephadex G 150 filtration. The specific activity
was 40 U/mg, and the purification fold was 25.6, yielding 51%. The cytotoxic
effect of the enzyme on treated cells was dose-dependent, with an IC50 value of
4.305 IU/ml.
Keywords: Cytotoxic effects, L-asparagine, Lactobacillus reuteri, breast cancer
cell line
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
Reference47 articles.
1. 1. Evans, D.; Howell, A. Breast cancer risk-assessment models. Br Cancer Res 2007, 9, 213.
2. 2. MAHDI, L, H; BAQER, B. AUDA. I. Anticarcinogenic, immuno modulatory and Antibacterial effect
3. of L-Argininedeiminase ADI against S. epidermidesCoNS mammary gland infection model.J. Int.J Pharma.Research., 2019, 11(2).
4. 3. Mahdi, L.; Mahdi, N.; Al-kakeia, S.; Musafera, H.; Al-Joofya, I.; Essaa, R.; Zwainb, L.; Salmana, I.;
5. Materc, H.; Al-Alaka, S.; Al-Oqailia, R. Treatment strategy by lactoperoxidase and lactoferrin combination:
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