Detection of Torque Teno virus in IRAQI hemodialysispatients by ELISA technique

Abstract

Background: Much evidence points to the presence of new factors that affect the liver in addition to the well-known hepatitis A-E viruses, and of these viruses is the Torque Teno virus, which was found to be more prevalent in the liver. 0bjecte: Detection of Torque Teno virus infection in patients undergoing dialysis with hepatitis C virus(HCV) patients and non-infected patients, comparing them with healthy people by ELISA technique to determine the extent of the virus spread in patients undergoing dialysis for the first time in the Iraqi community and how to affect the severity of infection. Methods: The study was conducted from 2021 until the end of 2022. Blood samples were collected from 35 patients infected with HCV undergoing hemodialysis, 35 patients not infected with HCV undergoing hemodialysis, and 20 healthy people. Clinical information and tests for hepatitis were obtained from the patients' registry. Chemical and hematological tests were done, and the results were recorded. A test for the detection of the Torque Teno virus was done by ELISA technology. Results: Torque Teno virus was detected in serum samples of patients using the ELISA technique. The infection rate was 0.0% in healthy people, (14.29%) in patients undergoing dialysis without HCV infection (5 out of 35) and (22.86%) in patients undergoing dialysis with HCV infection (8 out of 35), the novel result of this study showed that there is a higher prevalence of Torque Teno virus in HCV patients than in patients undergoing dialysis without HCV infection in Iraq. Also, this study found nonsignificant differences between Torque Teno virus infectionand liver function enzymes, neither with WBCs nor HB concentrations. Conclusion: Hepatitis may be caused by TTV, which was shown to be connected with biochemicalindicators of liver damage and persistent HBV or HCV infection. Keywords: Torque Teno virus, hemodialysis, ELISA technique