Affiliation:
1. Department of Biology, Faculty of Science, Ilam University, Ilam, Iran
Abstract
Effective, accurate, and rapid diagnostic tools for detecting SARS-Covid2 infection are urgently needed to prevent and control the pandemic. While RT-qPCR is
a gold standard test for diagnosing SARS-Covid2 infections, this method is limited
by the requirement for expensive equipment, experienced staff and specialized molecular laboratory. Recently, the CRISPR cas13 platform was used for the detection of COVID-19. This study evaluated the clinical performance of the CRISPRbased cas13a diagnostic assay for SARS-Covid2. In the current study, the diagnostic performance of the Cas13-based SHERLOCK (Specific High Sensitivity Enzymatic Reporter Unlocking) assay was compared with RT-qPCR in terms of sensitivity, specificity and time consumption.
Keywords: Crispr-Cas13; Covid-19; Sherlock
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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