In Vitro, Pathogenicity of Pseudomonas savasana Isolated from Olive Trees in Iraq on Fruits of Various Plant Species and its Detection by Polymerase Chain Reaction (BCR)

Abstract

In this study, after a field survey of olive trees in some northern areas in the Kurdistan region of Iraq and the province of Dohuk, samples were collected for bacteria Pseudomonas Savastonifrom olive trees infected with olive knot—the period from the beginning of March until the end of May for the diagnosis of Pseudomonas Savastoni. After activating the bacteria, we started inoculating the bacteria on the fruits of other plants, such as carrots, lemons, beans, local apples and hidden apples. These fruits were subjected to the same environmental conditions of humidity, temperature and incubation period. However, no symptoms of infection of the fruits with this bacteria appeared. Only the carrots were found to be infected with this bacteria after 13 days of incubation, but after 20 days, the bacteria had infected all the carrots. We isolated the bacteria again from the infected carrot. We performed all microscopic, phenotypic and biochemical tests and subjected the bacterial isolates to a device determined using the VITEK2 system to identify the type of bacteria. Bacteria we conducted molecular tests on them, such as the test PCR and the test RT-PCR for the gene 16S r RNA. After the results appeared, the isolates were subjected to genetic sequencing to ensure the occurrence of genetic mutations of the gene 16S r RNA. Keywords: Pseudomonas Savastoni, 16S r RNA, RT-PCR, VITEK2, Sequencing