Affiliation:
1. Genetic engineering and biotechnology institute, University of Baghdad. Iraq
2. Environment & Water Directorate/ Ministry of Science& Technology, Baghdad. Iraq
Abstract
The increasing resistance of Klebsiella pneumoniae to antibiotics has
led to difficulties in treating infections due to its virulence factors. As one of its
major pathogenic factors, this opportunistic pathogen may develop a thick biofilm coating, allowing the bacteria to attach to living or nonliving surfaces and
promote drug resistance. Searching for therapeutic alternatives from a plant
source that was safe and effective in treating this multi-drug-resistant bacteria
was necessary. In this concept, Syzygium aromaticum extract (SAE) is used to
combat K. pneumonia. The extract was confirmed by GC-MS and loaded onto
chitosan nanoparticles (SACSNPs). The SACSNPs were prepared by the ionic
gelation method with tripolyphosphate (TPP). And then characterized using UVvis, FTIR, AFM, SEM, and XRD techniques. The K. pneumonia isolates were
obtained and identified using the VITEK-2 system. The MIC of SAE and
SACSNPs were confirmed using a 96-well resazurin-aided microdilution method,
which was 6.25 μg/ml for SACSNPs and 75.5 μg/ml for SAE. The inhibitory activity using sub-MIC of analytical substances was determined by measuring the
optical density using a microplate reader with a 96-well plate and 0.1% crystal
violet dye. The results show that the S. aromaticum extract loaded with chitosan
nanoparticles has higher inhibitory activity against the biofilm formation of K.
pneumonia than the S. aromaticum extract.
Keywords: Chitosan nanoparticles, S. aromaticum, K. pneumonia, Biofilm, GCMS, resazurin, XRD.
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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