Preliminary Study of Polysaccharide and Oligosaccharide Alginate (AOS) as Prebiotic of Probiotic Bacteria

Abstract

Synbiotics are related to pre and probiotic mixture which work synergically and provide the intestine microbial balance in organisms. This study aims to evaluate the potency of alginate polysaccharide and oligosaccharide (AOS) as prebiotic to probiotic bacteria originally from a commercial yogurt. Probiotic bacteria were plated to MRSA and incubated in 24 hrs. Over five isolated bacteria, three probiotic isolate bacteria were defined positively(C11; C14 and C15). The prebiotic test was done by culturing the isolate C14 to MRSB and incubated 24 hrs. After 24 hrs, all the MRSB liquid culture became turbid, showed a positive effect, except the MRSB culture without any alginate enrichment. Probiotic bacteria from MRSB were then plated into MRSA for Total Plate Count (TPC) test and incubated. TPS revealed that 0.1 mg/mL enriched AOS media resulted the highest number of probiotic bacteria (1.193 x 105 CFU/mL) compared to enriched AOS and 0.05 mg AOS/mL media (6 x 103 CFU/mL). These results have proven a high ability to develop AOS as prebiotic.