Affiliation:
1. Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, India
Abstract
An enzyme-based drug, L-asparaginase, was produced byNocardia levisMK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature30∘C. Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase byN. levis.
Funder
Indian Council of Medical Research
Subject
General Environmental Science,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
13 articles.
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