Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula

Abstract

Simulium reptans(Linnaeus, 1758) andSimulium reptantoidesCarlsson, 1962 are two species of theSimulium reptansgroup whose distribution is unclear because of their confusing taxonomy and systematics. Their genetic variability is well known for populations in northern and central Europe and shows that both species have two forms; however, the genetic variability of these species in southern and eastern Europe is unknown. To identify the status of these two species in southeast Europe, mtDNA was extracted from 19 individuals from 12 localities across the Balkan Peninsula. Phylogenetic analysis confirmed the existence of two species with 7.38–7.94% divergence. Each species was comprised of two clades, with 2.31% and 1.43% interclade divergence forS. reptansandS. reptantoides, respectively. This study revealed the presence of both species across the Balkans and thatS. reptansoccurs in this area in only one form (S. reptansB), whileS. reptantoidesis found in two genetic forms (A and B).