Molecular genetics of Simulium (Wilhelmia) equinum (Linnaeus) (Diptera: Simuliidae) from West Siberia

Author:

VAULIN OLEG V.ORCID,PETROZHITSKAYA LIUDMILA V.ORCID,NOVGORODOVA TATIANA A.ORCID

Abstract

The medico-parasitological importance of bloodsucking black flies is high. To assess potential epidemiological risks and develop biological control programs for these bloodsucking Diptera, it is necessary to be informed of their diversity, community structure and the most common and dominant species. The degree of homogeneity of molecular genetic characteristics of one of the most widespread black fly species Simulium (W.) equinum (Linnaeus) in different parts of its range was investigated. The features of S. equinum from West Siberia where the species has become an abundant ecosystem component in recent years have been revealed. Analysis of species-diagnostic DNA-fragments (COI, ITS2 and D2 28S) in the specimens studied from this region, from the Novosibirsk Oblast (Russia) and data from the DNA database revealed four distinct branches tentatively referred to as West-European, East-European, Finland-Turkish, and Novosibirsk. Specimens from the Novosibirsk Branch were closer to the Finland-Turkish branch. We simulated restriction patterns of the standard COI fragment for all the branches. Substantial level of differences among the branches (4.1–14.8 % by COI sequences) raised the issue of their species status. A further study of S. equinum from West Siberia may clarify the ecological and medico-parasitological features of the Novosibirsk Branch of the species.  

Publisher

Magnolia Press

Subject

Animal Science and Zoology,Ecology, Evolution, Behavior and Systematics

Reference2 articles.

1.

Adler, P.H. (2020) World Blackflies (Diptera: Simuliidae): a comprehensive revision of the taxonomic and geographical inventory. Clemson University, Clemson, South Carolina, 142 pp.
Adler, P.H & McCreadie, J.W. (2019) Black Flies (Simuliidae). In: Mullen, G. & Durden, L. (Eds.), Medical and Veterinary Entomology. 3rd Edition. Academic Press, Boston, Massachusetts and Elsevier, Amsterdampp, pp. 23–259.
Adler, P.H., Cheke R. & Post R.J. (2010) Evolution, epidemiology and population genetics of black flies (Diptera: Simuliidae). Infection, Genetics and Evolution, 10 (7), 846–865. https://doi.org/10.1016/j.meegid.2010.07.003
Adler, P.H., Inci, A., Yildirim, A., Duzlu, O., McCreadie, J.W., Kúdela, M., Khazeni, A., Brúderová, T., Seitz, G., Takaoka, H., Otsuka, Y. & Bass, J. (2014) Are black flies of the subgenus Wilhelmia (Diptera: Simuliidae) multiple species or a single geographical generalist? Biological Journal of the Linnean Society, 114 (1), 16–183. https://doi.org/10.1111/bij.12403
Alburaki, M., Moulin, S., Legout, H., Alburaki, A. & Garnery, L. (2011) Mitochondrial structure of Eastern honeybee populations from Syria, Lebanon and Iraq. Apidologie, 42, 628–641. https://doi.org/10.1007/s13592-011-0062-4
Bender, W., Spierer, P., Hognes, D.S. & Chambon, P. (1983) Chromosomal walking and jumping to isolate DNA from Ace and rosy loci and the bithorax complex in Drosophila melanogaster. Journal of Molecular Biology, 168 (1), 17–33. https://doi.org/10.1016/s0022-2836(83)80320-9
Bernasconi, C., Pamilo, P. & Cherix, D. (2010) Molecular markers allow sibling species identification in red wood ants (Formica rufa group). Systematic Entomology, 35, 243–249. https://doi.org/10.1111/j.1365-3113.2009.00503.x
Bernotiene, R. & Stunţënas, V. (2009) On the biology of Simulium galeratum in Lithuania: ecological and molecular data. Ekolologija, 55 (2), 123–126. https://doi.org/10.2478/v10055-009-0015-7
Besansky, N.J., Powell, J.R., Caccone, A., Hamm, D.M., Scott, J.A. & Collins, F.H. (1994) Molecular phylogeny of the Anopheles gambiae complex suggests genetic introgression between principal malaria vectors. Proceedings of the National Academy of Sciences, 91 (15), 6885–6888. https://doi.org/10.1073/pnas.91.15.6885
Brower, A.V. (1994) Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proceedings of the National Academy of Sciences, 91 (14), 6491–6495. https://doi.org/10.1073/pnas.91.14.6491
Chubareva, L.A. & Bulli, V.A. (1983) The karyotypic analysis of the black fly Wilhelmia equina bianchii Rubz. (Diptera, Simuliidae) related to its taxonomical status. Cytology, 25 (5), 610–613.
Chubareva, L.A., Petrova, N.A. & Reva, M.V. (2007) Karyotypic and morphological study of five species of the genus Wilhelmia Enderlein (Diptera, Simuliidae). Entomological Review, 87(9), 1290–1299. https://doi.org/10.1134/S0013873807090187
Currie, D.C. & Adler, P.H. (2008) Global diversity of black flies (Diptera: Simuliidae) in freshwater. Hydrobiologia, 595, 469–75. https://doi.org/10.1007/s10750-007-9114-1
Day, J.C., Goodall, T.I. & Post, R.J. (2008) Confirmation of the species status of the blackfly Simulium galeratum in Britain using molecular taxonomy. Medical and Veterinary Entomology, 22, 55–61. https://doi.org/10.1111/j.1365-2915.2008.00719.x
Đuknić, J., Jovanović, V.M., Popović, N., Živić, I., Raković, M., Čerba, D. & Paunović, M. (2019) Phylogeography of Simulium subgenus Wilhelmia (Diptera: Simuliidae)—Insights from Balkan Populations. Journal of Medical Entomology, 56 (4), 967–978. https://doi.org/10.1093/jme/tjz034
Đuknić, J., Jovanović, V.M., Atlagić, J.Č., Andjus, S., Paunović, M., Živić, I. & Popović, N. (2020) Simulium reptans (Linnaeus, 1758) and Simulium reptantoides Carlsson, 1962 from the Balkan Peninsula. ZooKeys, 922, 141–155. https://doi.org/10.3897/zookeys.922.49306
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3 (5), 294–299.
Foster, W.A. & Walker, E.D. (2019) Mosquitoes (Culicidae). In: Mullen, G. & Durden, L. (Eds.), Medical and Veterinary Entomology. 3rd Edition. Academic Press, Boston, Massachusetts and Elsevier, Amsterdam, pp. 261–325.
Frey, J.E., Guillén, L., Frey, B., Samietz, J., Rull, J. & Aluja, M. (2013) Developing diagnostic SNP panels for the identification of true fruit flies (Diptera: Tephritidae) within the limits of COI-based species delimitation. BMC Evolutionary Biology, 13, 106. https://doi.org/10.1186/1471-2148-13-106
Goswami, G., Raghavendra, K., Nanda, N., Gakhar, S.K. & Subbarao, S.K. (2005) PCR-RFLP of mitochondrial cytochrome oxidase subunit II and ITS2 of ribosomal DNA: markers for the identification of members of the Anopheles culicifacies complex (Diptera: Culicidae). Acta Tropica, 95 (2), 92–99. https://doi.org/10.1016/j.actatropica.2005.04.018
Harbach, R.E. (2004) The classification of genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships. Bulletin of Entomological Research, 94 (6), 537–53. https://doi.org/10.1079/ber2004321
Hebert, P.D., Ratnasingham, S. & de Waard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences, 270 (Supplement 1), 96–99. https://doi.org/10.1098/rsbl.2003.0025
Hernández-Triana, L.M. (2007) Recommendations by The Natural History Museum on methods for collecting, preserving, rearing and mailing of simuliid specimens. Available from: http://blackflies.info/content/simuliidae-collecting-protocol (accessed 3 March 2020)
Inci, A., Yildrim, A., Duzlu, O., Onder, Z., Ciloglu, A., Seitz, G. & Adler, P.H. (2017) Genetic diversity and identification of Palearctic black flies in the subgenus Wilhelmia (Diptera: Simuliidae). Journal of Medical Entomology, 54 (4), 888–894. https://doi.org/10.1093/jme/tjw246
Ji, Y.J., Zhang, D. & He, L. (2003) Evolutionary conservation and versatility of a new set of primers for amplifying the ribosomal internal transcribed spacer regions in insects and other invertebrates. Molecular Ecology Notes, 3, 581–585. https://doi.org/10.1046/j.1471-8286.2003.00519.x.
Katz, A.D. (2020) Inferring evolutionary timescales without independent timing information: An assessment of “universal” insect rates to calibrate a Collembola (Hexapoda) molecular clock. Genes, 11 (10), 1172. https://doi.org/10.3390/genes11101172
Khanzadeh, F., Khaghaninia, S., Maleki-Ravasan, N., Oshaghi, M.A. & Adler, P.H. (2020). Black flies (Diptera: Simuliidae) of the Aras River Basin: Species composition and floral visitation. Acta Tropica, 209 (3), 105536. https://doi.org/10.1016/j.actatropica.2020.105536
Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120. https://doi.org/10.1007/bf01731581
Kúdela, M., Brúderová, T., Jedlička, L., Bernotienë, R., Celec, P. & Szemes, T. (2014) The identity and genetic characterization of Simulium reptans (Diptera: Simuliidae) from central and northern Europe. Zootaxa, 3802 (3), 301–17. https://doi.org/10.11646/zootaxa.3802.3.1
Kudryavtseva, T. Yu., Popov, V.P., Mokrievich, A.N., Pakskina, N.D., Kholin, A.V., Mazepa, A.V., Kulikalova, E.S., Kosilko, S.A., Birkovskaya, Yu.A., Trankvilevsky, D.V., Khramov, M.V. & Dyatlov, I.A. (2019) Epidemic activity of natural tularemia foci in the territory of the Russian Federation in 2018 and forecast of the situation for 2019. Problems of Particularly Dangerous Infections, 1, 32–41. [in Russian] https://doi.org/10.21055/0370-1069-2019-1-32-41
Lanzaro, G.C. & Lee, Y. (2013) Speciation in Anopheles gambiae—The distribution of genetic polymorphism and patterns of reproductive isolation among natural populations. In: Manguin, S. (Ed.), Anopheles mosquitoes—New Insights into Malaria Vectors. InTech. Publ., Rijeka, pp. 175–196.
Low, V.L., Takaoka, H., Adler, P.H., Ya’cob, Z., Norma-Rashid, Y., Chen, C.D. & Sofian-Azirun, M. (2015) A multi-locus approach resolves the phylogenetic relationships of the Simulium asakoae and Simulium ceylonicum species groups in Malaysia: evidence for distinct evolutionary lineages. Medical and Veterinary Entomology, 29, 330–337. https://doi.org/10.1111/mve.12120
Luchitskaya, I.O., Belaya, S.A. & Arbuzov, S.A. (2014) Climate of Novosibirsk and its changes. SB RAS, Novosibirsk, 224 pp.
Malmqvist, B., Adler, P.H., Kuusela, K., Merritt, R.W. & Wotton, R.S. (2004) Black flies in the boreal biome, key organisms in both terrestrial and aquatic environments: a review. Écoscience, 11 (2), 187–200. https://doi.org/10.1080/11956860.2004.11682824
Medvedev, S.G. & Aibulatov, S.V. (2012) The fauna of bloodsucking insects of the “gnus” complex (Diptera) in Leningrad Province and Saint-Petersburg. Parasitology, 46 (5), 350–368. [in Russian]
Mirzaeva, A.G. & Glushchenko, N.P. (2009) Bloodsucking Diptera in the Forest-Steppe Regions of Novosibirsk Province. Entomological Review, 89 (6), 659–671. https://doi.org/10.1134/S0013873809060049
Ney, G., Frederick, K. & Schul, J. (2018) A post-Pleistocene calibrated mutation rate from insect museum specimens. PLoS Currents, 2018, 10. https://doi.org/10.1371/currents.tol.aba557de56be881793261f7e1565cf35
Novikov, Yu. M & Shevchenko, A.I. (2001) Inversion polymorphism and the divergence of two cryptic forms of Anopheles messeae (Diptera, Culicidae) at the level of genomic DNA repeats. Russian Journal of Genetics, 37 (7), 754–763.
Papadopoulou, A., Anastasiou, I. & Vogler, A.P. (2010) Revisiting the insect mitochondrial molecular clock: The Mid-Aegean Trench Calibration. Molecular Biology and Evolution, 27 (7), 1659–1672. https://doi.org/10.1093/molbev/msq051
Patrusheva, V.D. (1966) Black flies (Simuliidae). In: Cherepanov, A.I. (Ed.), Biological bases for the control of black flies in the Ob basin. Nauka, Novosibirsk, pp. 53–117. [in Russian].
Patrusheva, V.D. (1982) Black flies of Siberia and the Far East. Nauka, Novosibirsk, 321 pp. [in Russian]
Petrozhitskaya, L.V. (2010) Black flies. In: Ravkin, Yu. S. (Ed.), Biodiversity of the Karasuksko-Burlinsky region (West Siberia). Siberian Branch of RAS, Novosibirsk, pp. 155–159. [in Russian]
Petrozhitskaya, L.V. (2013) Blood-sucking blackflies (Dipetra: Simuliidae). In: Zhimulev, I.F. (Ed.), Ecosystem dynamics in Novosibirsk Academgorodok. Nauka, Novosibirsk, pp. 278–282 [in Russian]
Petrozhitskaya, L.V. & Rodkina, V.I. (2018) Landscape–zonal distribution of blackflies (Diptera: Simuliidae) in the Ob-Irtysh River basin (Overview). Inland Water Biology, 11, 255–263. https://doi.org/10.1134/S1995082918020177
Tamura, K. (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678–687. https://doi.org/10.1093/oxfordjournals.molbev.a040752
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729. https://doi.org/10.1093/molbev/mst197
Saeung, A., Baimai, V., Otsuka, Y., Rattanarithikul, R., Somboon, P., Junkum, A., Tuetun, B., Takaoka, H. & Choochote, W. (2008). Molecular and cytogenetic evidence of three sibling species of the Anopheles barbirostris Form A (Diptera: Culicidae) in Thailand. Parasitology Research, 102 (3), 499–507. https://doi.org/10.1007/s00436-007-0788-0
Sedaghat, M.M., Linton, Y.M., Oshaghi, M.A., Vatandoost, H. & Harbach R.E. (2003) The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species. Bulletin of Entomological Research, 93 (6), 527–35. https://doi.org/10.1079/BER2003272
Shaikevich, E.V. (2007) PCR-RFLP of the COI gene reliably differentiates Cx. pipiens, Cx. pipiens f. molestus and Cx. torrentium of the Pipiens Complex. European Mosquito Bulletin, 23, 25–30.
Vobis, M., D’Haese, J., Mehlhorn, H., Mencke, N., Blagburn, B.L., Bond, R., Denholm, I., Dryden, M.W., Payne, P., Rust, M.K., Schroeder, I., Vaughn, M.B. & Bledsoe, D. (2004) Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers. Parasitology Research, 94, 219–226. https://doi.org/10.1007/s00436-004-1201-x
Rubtsov, I.A. (1962) Short guide to blood-sucking black flies of the fauna of the USSR. USSR Academy of Sciences Publishing House, Moscow-Leningrad, 228 pp. [in Russian]
Ruiz-Arrondo, I., Hernández-Triana, L.M., Ignjatović-Ćupina, A., Nikolova, N., Garza-Hernández, J.A., Rodríguez-Pérez, M.A., Oteo, J.A., Fooks, A.R. & Lucientes Curdi, J. (2018) DNA barcoding of blackflies (Diptera: Simuliidae) as a tool for species identification and detection of hidden diversity in the eastern regions of Spain. Parasites & Vectors, 11, 463. https://doi.org/10.1186/s13071-018-3046-7
Usova, Z.V. (1997) A checklist of Simuliidae (Diptera) of Leningrad Province. International Dipterological Resource, 8 (1), 23–24.
Vaulin, O.V. & Novikov, Yu. M. (2012) Polymorphism and interspecific variability of cytochrome oxydase subuniti (COI) gene nucleotide sequence in sibling species of A and B Anopheles messeae and An. beklemishevi (Diptera: Culicidae). Vavilov Journal of Genetics and Breeding, 16 (2), 358–368.
Vaulin, O.V. & Novikov, Yu. M. (2016) Phylogenetic relationships between Palaearctic species of the Anopheles maculipennis complex (Diptera: Culicidae) revealed by different approaches and markers. The problem of consensus. Vavilov Journal of Genetics and Breeding, 20 (5), 695–703. https://doi.org/10.18699/VJ16.189
Vaulin, O.V., Karagodin, D.A., Zakharov, I.K & Baricheva E.M. (2018) Dynamics of malaria mosquito species composition in Siberian populations detected by restriction analysis. Russian Journal of Genetics, 54, 838–847. https://doi.org/10.1134/S1022795418070153
Yankovsky, A.V. (2002) A key for the identification of blackflies (Diptera: Simuliidae) of Russia and adjacent countries (former USSR). Russian Academy of Sciences, St. Petersburg, 569 pp. [in Russian].

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