Affiliation:
1. Department of Dermatology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA
2. Section of Vascular Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA
3. Division of Cardiovascular Disease, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA
4. Englewood Hospital and Medical Center, Englewood, NJ
5. Department of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA
6. Section of Inflammation and Cardiometabolic Diseases, National Heart Lung and Blood Institute, Bethesda, MD
Abstract
Background
Psoriasis, especially when severe, is a risk factor for cardiometabolic disease beyond traditional risk factors. The mechanism of atherogenesis in psoriasis remains unknown. Cell membrane vesicles (ie, microparticles), released upon cell activation or apoptosis, have recently been associated with cardiometabolic disease and may play a pathogenic role. Microparticle levels, particularly from endothelial cells and platelets, are elevated in patients with cardiovascular disorders, metabolic syndrome, other inflammatory diseases, autoimmune conditions, and have been shown to be predictive of cardiovascular outcomes.
Methods and Results
Concentrations of microparticles with positive expression for any of 7 cell surface markers (Annexin V,
CD
3,
CD
31,
CD
41a,
CD
64,
CD
105, and
CD
144) were measured in blood samples from psoriasis patients (n=53) and control subjects without psoriasis (n=41). Platelet‐free plasma was separated from whole blood by one‐step centrifugation for microparticle analysis. Microparticles were fluorescently labeled and characterized by flow cytometry. Higher concentrations of
CD
105 (5.5/μL versus 2.5/μL,
P
<0.001),
CD
31 (31/μL versus 18/μL,
P
=0.002),
CD
41a (50/μL versus 22/μL,
P
<0.001), and
CD
64 (5.0/μL versus 4.1/μL,
P
=0.02) singly positive microparticles corresponding to endothelial cell‐, platelet‐, and monocyte/macrophage‐derived microparticles, respectively, were found in psoriasis patients compared with controls. These differences persisted after adjustment for traditional cardiometabolic risk factors including body mass index.
Conclusions
Increased microparticle concentrations, independent of cardiometabolic risk factors, in patients with psoriasis suggest that the presence of increased endothelial cell, platelet, and monocyte/macrophage activation with cell turnover may contribute to the heightened atherogenesis associated with psoriasis.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine
Cited by
56 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献