Affiliation:
1. From the Banting and Best Diabetes Centre, The Toronto Hospital Research Institute, and the Department of Pathology, University of Toronto, Ontario, Canada.
Abstract
Abstract
We have examined whether cell death contributes to postnatal remodeling of arteries in lambs. First, abdominal aortic smooth muscle cell proliferation rates fell from 2.87±0.08% per day at 3 days of age to 1.75±0.15% per day at 21 days. These proliferation rates would yield a 50% increase in DNA content in the absence of cell death. No increase in DNA content was observed (
P
<.05 for predicted versus measured accumulation); therefore, significant cell death was inferred. The same analysis did not indicate high cell-death rates in the carotid, renal, or iliac arteries; however, cell death was detected in situ by end-labeling partially degraded DNA with terminal deoxynucleotidyl transferase or by nuclear labeling with propidium iodide, a fluorescent dye that permeates only nonviable cells. Nuclei were labeled in all arteries, although labeling was most frequent in the abdominal aorta, a vessel that regresses substantially after birth. Cell death was apoptotic because DNA extracted from arteries and end-labeled with [
32
P]dCTP produced a series of low molecular weight bands (DNA ladder) on an agarose gel, a hallmark of apoptosis. The ladder was strong for neonatal abdominal aorta but weak for other arteries. Only weak laddering was observed for fetal abdominal aortas in late gestation, confirming that high apoptosis rates in this vessel were initiated after birth. Intense DNA ladders and frequent in situ labeling indicated high rates of apoptosis in the postnatal intra-abdominal umbilical artery, another vessel that regresses after birth. We conclude that apoptosis contributes to postpartum arterial remodeling . This contribution is greatest in arteries that regress after birth.
Publisher
Ovid Technologies (Wolters Kluwer Health)
Subject
Cardiology and Cardiovascular Medicine,Physiology
Reference41 articles.
1. Kerr JFR Harmon BV. Definition and incidence of apoptosis: an historical perspective. In: Tomei LD Cope FO eds. Apoptosis: The Molecular Basis of Cell Death . Plainview NY: Cold Spring Harbor Laboratory Press; 1991:5-29.
2. Apoptosis in metanephric development.
3. Programmed Cell Death and the Control of Cell Survival: Lessons from the Nervous System
4. Cell death programmed in uninduced metanephric mesenchymal cells
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