CHANGE OF INDICES OF THE AMINO ACID COMPOSITION OF RATS’ HEARTS AT ARTIFICIAL HYPOBIOSIS

Abstract

A series of unsolved questions in such sciences as: medicine, veterinary, biology still exist in the modern world. One of them is a search for new promising ways of anaesthetization, at which it would be unnecessary to use apparatuses as an “artificial heart”, “artificial ventilation of lungs” at short-term surgical interventions. Just artificial hypobiosis may become one of such methods. Main conditions for creation are a synchronous effect of such factors as hypoxia, hypercapnia, hypothermia. That is why for confirming the safety of this method in pre-clinical studies with a further perspective of using at clinical ones, it is necessary to study the mechanism of an effect and influence of the hypobiotic condition on the homeostasis of the living organism in detail. Rats are the best research object in this case. Just they have a similar physiological structure of such organs as a heart. An urgent question about changes that take place in the amino acid composition under the hypobiotic effect still be unexplained. That is why the aim of the study was to investigate amino acid changes of the rat heart under condition of artificial hypobiosis. White outbred male rats with mass 180–200 g were used in the experiments. The animals were divided in groups: control (intact) and experimental: the condition of artificial hypobiosis (first group) and 24 hours after release from artificial hypobiosis (second group). The number of animals in each group n=5. The experiments were conducted according to requirements of “The European convention about protection of vertebral animals, used with experimental or other scientific aims” (Strasbourg, France 1985), by general ethical principles of experiments with animals, approved by the First national congress of Ukraine on bioethics (2001). As a result of the conducted studies, a little decrease of several amino acids under condition of artificial hypobiosis was demonstrated. First of all, a decrease of such amino acids as aminosuccinic, glutamic, isoleucine, leucine, lysine, arginine was observed in rats’ hearths under artificial hypobiosis. There was also demonstrated an increase of the level of these amino acids in rats’ hearts after 24 hours after release from it.