A yeast FRET biosensor enlightens cAMP signaling-Reference-Cited by-同舟云学术

A yeast FRET biosensor enlightens cAMP signaling

Published:2021-06-15 Issue:13 Volume:32 Page:1229-1240
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Botman Dennis¹,O’Toole Tom G.²,Goedhart Joachim³,Bruggeman Frank J.¹,van Heerden Johan H.¹,Teusink Bas¹

Affiliation:

1. Systems Biology Lab/AIMMS, Vrije Universiteit Amsterdam, 1081 HV, Amsterdam, The Netherlands

2. Department of Molecular Cell Biology and Immunology, Vrije University Medical Center, 1081 HV Amsterdam, The Netherlands

3. Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands

Abstract

A FRET-based cAMP biosensor for in yeast showed that cAMP peaks differentiate between different carbon source transitions and are homogenous among single cells. Additionally, its height scales with the relative, and not the absolute, change in glucose. Last, the sensor showed that cAMP can convey information about prospective growth rates.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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