Photobleaching step analysis for robust determination of protein complex stoichiometries-Reference-Cited by-同舟云学术

Photobleaching step analysis for robust determination of protein complex stoichiometries

Published:2021-11-01 Issue:21 Volume:32 Page:
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Hummert Johan¹²³,Yserentant Klaus¹⁴²³,Fink Theresa¹,Euchner Jonas¹²³,Ho Yin Xin²³,Tashev Stanimir Asenov²³,Herten Dirk-Peter¹²³

Affiliation:

1. Institute of Physical Chemistry, Heidelberg University, D-69120 Heidelberg, Germany

2. Institute of Cardiovascular Sciences, College of Medical and Dental Sciences & School of Chemistry, University of Birmingham, Birmingham, B152TT UK

3. Centre of Membrane Proteins and Receptors (COMPARE), The Universities of Birmingham and Nottingham, The Midlands, Birmingham, B15 2TT UK

4. Faculty of Biosciences, Heidelberg University, D-69120 Heidelberg, Germany

Abstract

A reliable and automated single-molecule photobleaching step analysis is presented for protein copy number quantification in complexes containing up to 35 labeled proteins in situ. A combined approach for robust and efficient data analysis and experimental calibration is used.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

Reference52 articles.

1. Principles of assembly reveal a periodic table of protein complexes

2. An Oxygen Scavenging System for Improvement of Dye Stability in Single-Molecule Fluorescence Experiments

3. Deciphering the Subunit Composition of Multimeric Proteins by Counting Photobleaching Steps

4. Estimating numbers of intracellular molecules through analysing fluctuations in photobleaching

Cited by 25 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Advanced multiparametric image spectroscopy and super-resolution microscopy reveal a minimal model of CD95 signal initiation;Science Advances;2024-08-30

2. ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting;Nature Methods;2024-08-08

3. Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actin;2024-07-01

4. Multicolor, Cell-Impermeable, and High Affinity BACE1 Inhibitor Probes Enable Superior Endogenous Staining and Imaging of Single Molecules;Journal of Medicinal Chemistry;2024-06-06

5. Fluorescence microscopy: A statistics-optics perspective;Reviews of Modern Physics;2024-06-05