New Components of a System for Phosphate Accumulation and Polyphosphate Metabolism inSaccharomyces cerevisiaeRevealed by Genomic Expression Analysis

Author:

Ogawa Nobuo1,DeRisi Joseph2,Brown Patrick O.1

Affiliation:

1. Department of Biochemistry, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305-5307; and

2. Department of Biochemistry and Biophysics, University of California at San Francisco, 513 Parnassus Ave., San Francisco, California 94143

Abstract

The PHO regulatory pathway is involved in the acquisition of phosphate (Pi) in the yeast Saccharomyces cerevisiae. When extracellular Piconcentrations are low, several genes are transcriptionally induced by this pathway, which includes the Pho4 transcriptional activator, the Pho80-Pho85 cyclin-CDK pair, and the Pho81 CDK inhibitor. In an attempt to identify all the components regulated by this system, a whole-genome DNA microarray analysis was employed, and 22 PHO-regulated genes were identified. The promoter regions of 21 of these genes contained at least one copy of a sequence that matched the Pho4 recognition site. Eight of these genes, PHM1–PHM8, had no previously defined function in phosphate metabolism. The amino acid sequences ofPHM1 (YFL004w), PHM2 (YPL019c),PHM3 (YJL012c), and PHM4 (YER072w) are 32–56% identical. The phm3 and phm4single mutants and the phm1 phm2 double mutant were each severely deficient in accumulation of inorganic polyphosphate (polyP) and Pi. The phenotype of thephm5 mutant suggests that PHM5 (YDR452w)is essential for normal catabolism of polyP in the yeast vacuole. Taken together, the results reveal important new features of a genetic system that plays a critical role in Piacquisition and polyP metabolism in yeast.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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