Effects of the microtubule nucleator Mto1 on chromosomal movement, DNA repair, and sister chromatid cohesion in fission yeast

Author:

Zhurinsky Jacob1,Salas-Pino Silvia1,Iglesias-Romero Ana B.1,Torres-Mendez Antonio1,Knapp Benjamin12,Flor-Parra Ignacio1,Wang Jiyong3,Bao Kehan3,Jia Songtao3,Chang Fred12,Daga Rafael R.1

Affiliation:

1. Centro Andaluz de Biologia del Desarrollo, Universidad Pablo de Olavide, Seville 41013, Spain

2. Department of Microbiology and Immunology, Columbia University College of Physicians and Surgeons, New York, NY 10032

3. Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143

Abstract

Although the function of microtubules (MTs) in chromosomal segregation during mitosis is well characterized, much less is known about the role of MTs in chromosomal functions during interphase. In the fission yeast Schizosaccharomyces pombe, dynamic cytoplasmic MT bundles move chromosomes in an oscillatory manner during interphase via linkages through the nuclear envelope (NE) at the spindle pole body (SPB) and other sites. Mto1 is a cytoplasmic factor that mediates the nucleation and attachment of cytoplasmic MTs to the nucleus. Here, we test the function of these cytoplasmic MTs and Mto1 on DNA repair and recombination during interphase. We find that mto1Δ cells exhibit defects in DNA repair and homologous recombination (HR) and abnormal DNA repair factory dynamics. In these cells, sister chromatids are not properly paired, and binding of Rad21 cohesin subunit along chromosomal arms is reduced. Our findings suggest a model in which cytoplasmic MTs and Mto1 facilitate efficient DNA repair and HR by promoting dynamic chromosomal organization and cohesion in the nucleus.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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