Conformational diversity of dynactin sidearm and domain organization of its subunit p150-Reference-Cited by-同舟云学术

Conformational diversity of dynactin sidearm and domain organization of its subunit p150

Published:2020-06-01 Issue:12 Volume:31 Page:1218-1231
ISSN:1059-1524
Container-title:Molecular Biology of the Cell
language:en
Short-container-title:MBoC

Author:

Saito Kei¹,Murayama Takashi²,Hata Tomone¹,Kobayashi Takuya²,Shibata Keitaro¹,Kazuno Saiko³,Fujimura Tsutomu³,Sakurai Takashi²,Toyoshima Yoko Y.¹⁴

Affiliation:

1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan

2. Department of Cellular and Molecular Pharmacology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan

3. Laboratory of Proteomics and Biomolecular Science, Biomedical Research Center, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan

4. Komaba Institute for Science, Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan

Abstract

Using nanogold labeling and deletion mutant analysis, we determined the domain organization of dynactin subunit p150 and discovered that its CC1 domain adopted either a folded or an extended form. Furthermore, the entire sidearm of dynactin exhibited several characteristic forms, and the equilibrium among them depended on salt concentrations.

Publisher

American Society for Cell Biology (ASCB)

Subject

Cell Biology,Molecular Biology

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