YABANMERSİNİ (VACCİNİUM MYRTİLLUS L.) MEYVE ÖZÜ'NÜN MİDE KANSERİ, AGS HÜCRE HATTI ÜZERİNDE ANTİOKSİDAN AKTİVİTESİ VE ANTİ-KANSER ETKİLERİ
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Published:2022-09-27
Issue:
Volume:
Page:781-791
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ISSN:1015-3918
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Container-title:Ankara Universitesi Eczacilik Fakultesi Dergisi
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language:en
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Short-container-title:Ankara Ecz. Fak. Derg.
Author:
KARAKAŞ Nihal1, OKUR Mehmet Evren2, SAĞIR Tuğba3, ULUDAĞ Damla1, POLAT Derya Çiçek4, KARADAĞ Ayşe Esra5
Affiliation:
1. ISTANBUL MEDIPOL UNIVERSITY 2. UNIVERSITY OF HEALTH SCIENCES 3. Pim Grup Cosmetics Consultancy 4. ANKARA UNIVERSITY 5. ISTANBUL MEDIPOL UNIVERSITY, FACULTY OF PHARMACY
Abstract
Objective: Vaccinium myrtillus L. fruits are consumed as food. This research was aimed to evaluate V. myrtillus methanol extract antioxidant and cytotoxic activities and determine its anti-cancer potential to further study against gastric cancer. Material and Method: V. myrtillus fruit (Bilberry) methanol extract was examined for its antioxidant activities by ABTS•+ and DPPH• assays. The phytochemical analysis of the extract was studied by HPLC method. The cytotoxic effect of V. myrtillus fruit methanol extract on gastric cancer cell line AGS was measured by Cell Titer-Glo assay. Additionally, as healthy control, fibroblast like human mesenchymal stem cell line was used for testing anti-cancer efficacy. Result and Discussion: V. myrtillus fruit methanol extract showed 0.1413 and 0.0439 mg/mL IC50 values as antioxidant activity by ABTS•+ and DPPH• assays, respectively. Malvidin-3-O-Glucoside was detected as an anthocyanin compound by HPLC method. Cytotoxicity analysis showed that among different concentrations (0.5-10 mg/ml), the most significantly, 2 mg/ml of Bilberry extract treatment decreased the viability of AGS gastric cancer cells while sparing healthy MSC cells. This data suggests the further analysis of Bilberry extract on several cancer cell lines as well as the determination of a potential active substance in the extract.
Publisher
Ankara Universitesi Eczacilik Fakultesi Dergisi
Subject
General Earth and Planetary Sciences,General Environmental Science
Reference42 articles.
1. 1. Baytop, T. (1999). Türkiyede Bitkiler ile Tedavi, Nobel Tıp Kitabevleri, Istanbul. 2. 2. Bujor, O.C., Le Bourvellec, C., Volf, I., Popa, V.I., Dufour, C. (2016). Seasonal variations of the phenolic constituents in bilberry (Vaccinium myrtillus L.) leaves, stems and fruits, and their antioxidant activity. Food Chemistry, 213, 58–68. [CrossRef] 3. 3. Güner, A. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler), Nezahat Gökyiğit Botanik Bahçesi Yayınları, Istanbul. 4. 4. Rouanet, J.M., Décordé, K., Rio, D.D. , Auger, C., Borges, G., Cristol, J.P., Lean M.E.J., Crozier, A. (2010). Berry juices, teas, antioxidants and the prevention of atherosclerosis in hamsters. Food Chemistry, 118(2), 266–271. [CrossRef] 5. 5. Ochkur, O., Del Bubba, M., Orlandini, S., Kravchenko, I., Koshovyi, O., Checchini, L., Bohachik, J. (2019). Study on the Polyphenols and Antocyanins Content In the Vaccinium myrtillus L. fruits harvested in different regions of Ukraine. Electronic Archive of the National Pharmaceutical University, 285–287. [CrossRef]
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