Affiliation:
1. Plant Pathology Department, University of Wisconsin, Madison, Wisconsin 53706
Abstract
ABSTRACT
The physicochemical forces that mediate attachment of yeasts to the phylloplane are unknown. Cell surface charge and hydrophobicity and adhesion to polystyrene, glass, and barley were assessed for wild-type
Rhodosporidium toruloides
and attachment-minus (Att
−
) mutants. Cells were grown under conditions promoting (excess carbon) or not promoting (excess nitrogen) capsule production. Hydrophobicity was measured by adhesion to xylenes, and surface charge characteristics were assessed by attachment to either DEAE (positive)- or carboxymethyl (CM) (negative)-Sephadex ion-exchange beads. Hydrophobicity and adhesiveness of nonencapsulated, wild-type
R. toruloides
decreased from mid-log to late stationary phase. Encapsulated wild-type
R. toruloides
cells were more hydrophobic and more adhesive than nonencapsulated cells. However, two encapsulated Att
−
mutants were more hydrophobic than the wild type and levels of adhesion of
R. toruloides
were similar on polystyrene and less hydrophobic glass surfaces. Adhesion of wild-type yeast to barley and polystyrene was correlated with attachment to CM-Sephadex beads, indicating a positive cell surface charge. Sixteen Att
−
mutants did not exhibit a positive cell surface charge, and wild-type yeast cells that did not attach to CM-Sephadex did not adhere to either polystyrene or barley. Wild-type
R. toruloides
attached to CM-Sephadex beads by the poles of the cells, indicating a localization of positive charge which was also visualized with India ink. We conclude that localized, positive charge, and not hydrophobic interactions, mediates attachment of
R. toruloides
to barley leaves.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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