Affiliation:
1. Department of Biology, University of Utah, Salt Lake City, Utah 84112
Abstract
ABSTRACT
Mutants of
Salmonella enterica
lacking polyphosphate kinase (
ppk
) grow poorly in the presence of the weak organic acids acetate, propionate, and benzoate. This sensitivity is corrected by methionine and seems to result from destabilization of MetA (homoserine transsuccinylase), the first enzyme in methionine biosynthesis. The MetA protein is known to be sensitive to thermal inactivation, and
ppk
mutants are more sensitive to heat-induced methionine auxotrophy. Peroxide increases the sensitivity of
ppk
mutants to both heat and acid and may oxidatively damage (carbonylate) destabilized MetA. While acid appears to impair methionine biosynthesis, it leads to derepression of MetA and may inhibit growth by causing toxic accumulation of denatured protein. This is supported by the observation that the overexpression of MetA in
ppk
mutants causes acid sensitivity that is not corrected by methionine. We propose that polyphosphate acts as a chemical chaperone that helps refold MetA and/or may stimulate proteolysis of toxic denatured protein. The instability of MetA protein may provide a metabolic fuse that blocks growth under conditions that denature proteins; the sensitivity of this fuse is modulated by polyphosphate.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
40 articles.
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