A Novel Real-Time PCR-Based Screening Test with Pooled Fecal Samples for Bovine Johne’s Disease-Reference-Cited by-同舟云学术

A Novel Real-Time PCR-Based Screening Test with Pooled Fecal Samples for Bovine Johne’s Disease

Published:2020-11-18 Issue:12 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kawaji Satoko¹^ORCID,Nagata Reiko¹,Minegishi Yasutaka²,Saruyama Yumi³,Mita Akiko⁴,Kishizuka Shingo⁴,Saito Masahiro⁴,Mori Yasuyuki¹

Affiliation:

1. Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Japan

2. Nippon Gene Co., Ltd., Tokyo, Japan

3. Tochigi Prefectural Central Livestock Hygiene Service Center, Utsunomiya, Japan

4. National Livestock Breeding Center, Nishigo, Japan

Abstract

Johne’s disease (JD) is an economically important infectious disease in livestock farming caused by Mycobacterium avium subsp. paratuberculosis . As an alternative to serological tests, which are used mainly for the screening of whole herds, we developed a novel ResoLight-based real-time PCR (RL-PCR) assay with pooled fecal samples for the detection of fecal shedders in cattle herds. The RL-PCR assay included an internal amplification control (IC) which was amplified using the same primer pair as the target molecule M. avium subsp.

Funder

NARO | Bio-oriented Technology Research Advancement Institution

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01761-20

Reference34 articles.

1. A review of bovine Johne's disease control activities in 6 endemically infected countries

2. Epidemiologic Indicators Associated with Within-farm Spread of Johne's Disease in Dairy Farms in Japan

3. Control of paratuberculosis: who, why and how. A review of 48 countries