Affiliation:
1. Soil Microbiology, Agronomy Department, Oklahoma State University, Stillwater, Oklahoma 74074
Abstract
A method is presented to identify esterase-synthesizing microbial colonies within mixed culture plates by utilizing induced esterase hydrolysis of nonfluorescent butyryl ester of 7-hydroxy-4-methylcoumarin to the highly fluorescent 7-hydroxy-4 methyl umbelliferone. Microscopy procedures for making esterase loci of fungal mycelia visible with this reaction are described.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
4 articles.
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