Affiliation:
1. Hygiene Institute, University of Vienna, Austria.
Abstract
Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities. By using these techniques, enzymatic reactions can be examined simultaneously or individually, either directly on the isolation plate or in cell suspensions. For this purpose, various testing principles and test kits for clinical and food microbiology have been introduced successfully during the last few years. In this paper we present a survey of different enzymes of microbial origin that are utilized for microbiological identification and differentiation and the corresponding methods. Particular emphasis is given to the examination of Escherichia coli and the description of the different techniques as used in routine analysis.
Publisher
American Society for Microbiology
Subject
Applied Microbiology and Biotechnology
Reference247 articles.
1. Colorimetric enumeration of Escherichia coli based on ,-glucuronidase activity;Adams M. R.;Appl. Environ. Microbiol.,1990
2. Rapid fluorescence method for screening Salmonella spp. from enteric differential agars;Aguirre P. M.;J. Clin. Microbiol.,1990
3. Temperature-induced alterations in 8-anilino-1-naphthalenesulfonate fluorescences with membranes from Mycobacterium phlei;Aithal H. N.;Biochemistry,1974
4. Reaktionen verschiedener Enterobacteriaceae- und Vibrionaceae-Spezies in BRILA-MUG (Fluorocult)-Bouillon;Aleksik S.;Zentralbl. Hyg.,1990
5. The enzymatic activity associated with Tamm-Horsfall glycoprotein;Alexander D. P.;IRCS Med. Sci.,1984
Cited by
74 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献