Affiliation:
1. Microbiology Division, Food Laboratory, U.S. Army Natick Laboratories, Natick, Massachusetts 01760
Abstract
A biphasic culture medium suitable for cultivation and sporulation of
Clostridium perfringens, C. botulinum
, and
C. sporogenes
was devised. The medium designed for use in a disposable, compartmented, plastic film container contained peptones, yeast extract, minerals, an anion exchange resin, and glucose in 4% agar as the solid phase and (NH
4
)
2
SO
4
and 0.1% agar as the liquid phase. With the biphasic system, it was not necessary to use active cultures as inocula. Growth was at least equal to that obtained in conventional media, and spore production of 9 out of 12 strains of
C. perfringens
equalled or usually exceeded that of conventional media.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference18 articles.
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