Methyl Violet: a Selective Agent for Differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and Other Gram-Negative Organisms

Author:

Campbell Lucy M.1,Roth Ivan L.1

Affiliation:

1. Department of Microbiology, The University of Georgia, Athens, Georgia 30602

Abstract

Three selective media for differentiation of Klebsiella pneumoniae from Enterobacter aerogenes on the basis of colonial morphology were evaluated. Using methyl violet 2B as a selective agent, strains of K. pneumoniae isolated from urine, fresh water, and fresh produce were tested against other members of Enterobacteriaceae in addition to strains of Aeromonas hydrophila and Pseudomonas aeruginosa . Comparison of colonial morphology showed K. pneumoniae produced larger, smoother colonies than other bacteria tested. These media were developed to aid in presumptive separation of K. pneumoniae from E. aerogenes in the monitoring of bacterial quality of water.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference14 articles.

1. American Public Health Association. 1971. Standard methods for the examination of water and wastewater 13th ed. American Public Health Association Inc. New York.

2. Bordner R. H. and B. J. Carroll. 1972. Proceedings of seminar on the significance of fecal coliform in industrial wastes. U.S. Environmental Protection Agency Denver Colo.

3. Buchanan R. E. and N. E. Gibbons. 1974. Bergey's manual of determinative bacteriology 8th ed. The Williams & Wilkins Co. Baltimore.

4. Conn H. J. 1961. Biological stains. The Williams & Wilkins Co. Baltimore.

5. Klebsiella biotypes among coliforms isolated from forest environments and farm produce;Duncan D. W.;Appl. Microbiol.,1972

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. The Genus Klebsiella;The Prokaryotes;2006

2. Enterobacteriaceae associated with meats and meat handling;Applied and Environmental Microbiology;1981-04

3. Primary Klebsiella identification with MacConkey-inositol-carbenicillin agar;Applied and Environmental Microbiology;1978-09

4. Evaluation of double violet agar in the isolation of Klebsiella pneumoniae from river water;Applied and Environmental Microbiology;1976-02

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3