Performance of PCR/Electrospray Ionization-Mass Spectrometry on Whole Blood for Detection of Bloodstream Microorganisms in Patients with Suspected Sepsis

Author:

Strålin Kristoffer12ORCID,Rothman Richard E.3,Özenci Volkan45,Barkataki Kieron6,Brealey David7,Dhiman Neelam8,Poling Lara9,Kurz Michael C.10,Limaye Ajit P.11,LoVecchio Frank12,Lowery Kristin9,Miller Loren G.13,Moran Gregory J.14,Overcash J. Scott15,Parekh Amisha16,Peacock W. Frank17,Rivers Emanuel P.1819,Sims Matthew20,Stubbs Amy M.21,Sundqvist Martin22,Ullberg Måns45,Carroll Karen C.23

Affiliation:

1. Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden

2. Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden

3. Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

4. Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden

5. Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

6. Department of Emergency Medicine, Kern Medical Center, Bakersfield, California, USA

7. Division of Critical Care, NIHR Biomedical Research Centre at University of College London Hospitals NHS Foundation Trust, London, United Kingdom

8. Molecular Infectious Diseases, med fusion/Quest Diagnostics, Lewisville, Texas, USA

9. AthoGen Testing, Carlsbad, California, USA

10. Department of Emergency Medicine, University of Alabama School of Medicine, Birmingham, Alabama, USA

11. University of Washington, Seattle, Washington, USA

12. University of Arizona, Maricopa Medical Center, Phoenix, Arizona, USA

13. Lundquist Institute at Harbor-UCLA Medical Center, Torrance, California, USA

14. Department of Emergency Medicine, Olive View-UCLA Medical Center, Sylmar, California, USA

15. eStudySite, Sharp Chula Vista, San Diego, California, USA

16. Department of Emergency Medicine, New York Methodist Hospital, New York, New York, USA

17. Baylor College of Medicine, Houston, Texas, USA

18. Department of Emergency Medicine and Surgery, Henry Ford Hospital, Detroit, Michigan, USA

19. Department of Surgery, Henry Ford Hospital, Detroit, Michigan, USA

20. Beaumont Health, Royal Oak and Oakland University William Beaumont School of Medicine, Rochester, Michigan, USA

21. Department of Emergency Medicine, Truman Medical Center, University of Missouri—Kansas City, Kansas City, Missouri, USA

22. Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden

23. Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Abstract

Blood culture (BC) often fails to detect bloodstream microorganisms in sepsis. However, molecular diagnostics hold great potential. The molecular method PCR/electrospray ionization-mass spectrometry (PCR/ESI-MS) can detect DNA from hundreds of different microorganisms in whole blood. The aim of the present study was to evaluate the performance of this method in a multicenter study including 16 teaching hospitals in the United States ( n  = 13) and Europe ( n  = 3).

Funder

Ibis Biosciences

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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