Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases-Reference-Cited by-同舟云学术

Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases

Published:2022-12-21 Issue:6 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Chen Lu¹,Zhao Yujuan¹,Wei Jiakai¹,Huang Wendi¹,Ma Ying¹,Yang Xuefeng²,Liu Yang¹,Wang Jing³,Xia Han³,Lou Zheng³^ORCID

Affiliation:

1. Xi’an Children’s Hospital, Xi’an, China

2. Neonatal Intensive Care Department, Xi’an Children’s Hospital, Xi’an, China

3. Department of Scientific Affairs, Hugobiotech Co., Ltd., Beijing, China

Abstract

To the best of our knowledge, this is the first report on evaluating the performance of mNGS using cfDNA and RNA from body fluid and blood samples for diagnosing neonatal infections. mNGS of RNA and cfDNA can achieve the unbiased detection and identification of trace pathogens from different kinds of neonatal body fluid and blood samples with a high total coincidence rate (226/331; 68.3%) against final clinical diagnoses by sample. The best timing for mNGS detection in neonatal infections ranged from 1 to 3 days, rather than 0 days, after the start of continuous antimicrobial therapy.

Funder

Beijing Municipal Science and Technology Commission

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.01195-22

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