Affiliation:
1. Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Federal Republic of Germany.
Abstract
Oligonucleotides specific at a genus, group, or species level were defined by a systematic comparison of small-subunit rRNA sequences from Mycobacterium tuberculosis, M. bovis, M. africanum, M. bovis BCG, M. avium, M. kansasii, M. marinum, M. gastri, M. chelonae, M. smegmatis, M. terrae, M. nonchromogenicum, M. xenopi, M. malmoense, M. szulgai, M. scrofulaceum, M. fortuitum, M. gordonae, M. intracellulare, M. simiae, M. flavescens, M. paratuberculosis, M. sphagni, M. cookii, M. komossense, M. phlei, and M. farcinica. On the basis of the defined oligonucleotides, the polymerase chain reaction technique was explored to develop a sensitive taxon-specific detection system for mycobacteria. By using M. tuberculosis as a model system, fewer than 10 bacteria could be reliably detected by this kind of assay. These results suggest that amplification of rRNA sequences by the polymerase chain reaction may provide a highly sensitive and specific tool for the direct detection of microorganisms without the need for prior cultivation.
Publisher
American Society for Microbiology
Reference26 articles.
1. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1987. Current protocols in molecular biology. John Wiley & Sons Inc. New York.
2. Diagnosis of tuberculosis;Bates J. H.;Chest,1979
3. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA;Bottger E. C.;FEMS Microbiol. Lett.,1989
4. Reclassification of Arachnia propionica as Propionibacterium propionicus comb. nov;Charfreitag O.;Int. J. Syst. Bacteriol.,1988
5. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae;Clark-Curtiss J. E.;J. Bacteriol.,1985