Identification of Candida species by PCR and restriction fragment length polymorphism analysis of intergenic spacer regions of ribosomal DNA

Author:

Williams D W1,Wilson M J1,Lewis M A1,Potts A J1

Affiliation:

1. Department of Oral Surgery, Medicine, and Pathology, Dental School, University of Wales College of Medicine, Cardiff, United Kingdom.

Abstract

The PCR was used to amplify a targeted region of the ribosomal DNA from 84 Candida isolates. Unique product sizes were obtained for Candida guilliermondii, Candida (Torulopsis) glabrata, and Candida pseudotropicalis. Isolates of Candida albicans, Candida tropicalis, Candida stellatoidea, Candida parapsilosis, and Candida krusei could be identified following restriction digestion of the PCR products.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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