Physical map of the conjugal plasmid of Neisseria gonorrhoeae

Author:

Tenover F C,Mayer L W,Young F E

Abstract

The 24.5-megadalton plasmid of Neisseria gonorrhoeae is required for transfer of R-factors and possibly chromosomal markers during conjugal matings between gonococcal strains. We constructed a physical map of one such plasmid, pLE2451, using EcoRI, BglII, and HincII site-specific restriction endonucleases. The patterns of deoxyribonucleic acid digestion obtained with this plasmid were identical to those obtained with three other plasmids of similar size.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference16 articles.

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2. Conjugal transfer of the gonococcal penicillinase plasmid;Eisenstein B. I.;Science,1977

3. Elwell L. P. and S. Falkow. 1977. Plasmids of the genus Neisseria p. 137-154. In R. B. Roberts (ed.) The gonococcus. John Wiley & Sons Inc. New York.

4. Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae;Elwell L. P.;Antimicrob. Agents Chemother.,1977

5. Chromosome mobilization by the R plasmid R68.45: a tool in Pseudomonas genetics;Haas D.;Mol. Gen. Genet.,1978

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