Affiliation:
1. Biochemistry Department, John Curtin School of Medical Research, The Institute of Advanced Studies, Australian National University, Canberra, A.C.T. Australia
Abstract
A mutant of
Escherichia coli
K-12 unable to form an essential component of the enterochelin-dependent iron transport system has been isolated. This strain carries a mutation in a gene designated
fep
, mapping close to two genes,
entA
and
entD
, concerned with enterochelin synthesis. Strain AN102, which carries the
fep
−
allele, accumulates large quantities of enterochelin and gives a growth response to sodium citrate. The cytochrome
b
1
and total iron content, and the measurement of the uptake of
55
Fe
3+
, indicate an impairment of the enterochelin-dependent iron transport system. The growth response to sodium citrate is related to the presence, in strain AN102, of an inducible citrate-dependent iron transport system.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
136 articles.
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