Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein-Reference-Cited by-同舟云学术

Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein

Published:2021-12-22 Issue:3 Volume:9 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Davis James J.¹²^ORCID,Long S. Wesley³⁴⁵^ORCID,Christensen Paul A.³⁴⁵,Olsen Randall J.³⁴⁵,Olson Robert¹²,Shukla Maulik¹²,Subedi Sishir³⁴⁵,Stevens Rick⁶⁷,Musser James M.³⁴⁵

Affiliation:

1. Division of Data Science and Learning, Argonne National Laboratory, Lemont, Illinois, USA

2. University of Chicago Consortium for Advanced Science and Engineering, Chicago, Illinois, USA

3. Center for Infectious Diseases, Laboratory of Molecular and Translational Human Infectious Diseases Research, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute and Houston Methodist Hospital, Houston, Texas, USA

4. Departments of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA

5. Departments of Microbiology and Immunology, Weill Cornell Medical College, New York, New York, USA

6. Computing, Environment and Life Sciences Directorate, Argonne National Laboratory, Argonne, Illinois, USA

7. Department of Computer Science, University of Chicago, Chicago, Illinois, USA

Abstract

ARTIC Network primers are commonly used by laboratories worldwide to amplify and sequence SARS-CoV-2 present in clinical samples. As new variants have evolved and spread, it was found that the V3 primer set poorly amplified several key mutations.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/Spectrum.01803-21

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