Thiamine-dependent Accumulation of Tetramethylpyrazine Accompanying a Mutation in the Isoleucine-Valine Pathway

Author:

Demain A. L.1,Jackson M.1,Trenner N. R.1

Affiliation:

1. Merck Sharp & Dohme Research Laboratories, Merck & Co., Inc., Rahway, New Jersey 07065

Abstract

A mutant of Corynebacterium glutamicum was found to accumulate high concentrations of a material which crystallized upon cooling of the broth. The compound was identified as tetramethylpyrazine. The mutant was found to require isoleucine, valine, leucine, and pantothenate for growth. All four requirements probably result from the loss of a single enzyme of the isoleucine-valine pathway. Since similar mutants of Neurospora crassa accumulate acetoin, the present mutant probably forms tetramethylpyrazine from acetoin. Accumulation of tetramethylpyrazine was dependent upon addition of thiamine. This observation is consistent with the known activity of diphosphothiamine as a cofactor for the formation of acetolactate (a precursor of acetoin) from pyruvate.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference11 articles.

1. Studies on the metabolic products of Bacillus subtilis. IV. Determination and mechanisms of formation of tetramethylpyrazine;ADACHI T., H.;J. Pharm. Soc. Japan,1964

2. Evidence for two distinct enzyme systems forming acetolactate in Aerobacter aerogenes;HALPERN Y. S.;J. Biol. Chem.,1959

3. KINOSHrIA S. 1963. Amino acids p. 206-226. In C. Rainbow and A. H. Rose [ed.] Biochemistry of industrial microorganisms. Academic Press Inc. New York.

4. The stereoisomeric 2:3:5:6- tetramethyl piperazines. I;KIPPING F. B.;J. Chem. Soc., p.2889-2897.,1929

5. KLEIN B. AND J. BERKOWITZ. 1959. Pyrazines. J. BAC-TERIOL.

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