Affiliation:
1. Merck Sharp & Dohme Research Laboratories, Merck & Co., Inc., Rahway, New Jersey 07065
Abstract
A mutant of
Corynebacterium glutamicum
was found to accumulate high concentrations of a material which crystallized upon cooling of the broth. The compound was identified as tetramethylpyrazine. The mutant was found to require isoleucine, valine, leucine, and pantothenate for growth. All four requirements probably result from the loss of a single enzyme of the isoleucine-valine pathway. Since similar mutants of
Neurospora crassa
accumulate acetoin, the present mutant probably forms tetramethylpyrazine from acetoin. Accumulation of tetramethylpyrazine was dependent upon addition of thiamine. This observation is consistent with the known activity of diphosphothiamine as a cofactor for the formation of acetolactate (a precursor of acetoin) from pyruvate.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference11 articles.
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