Affiliation:
1. Department of Pathobiology, University of Washington, Seattle, Washington 98195
2. School of Dentistry, University of Lisbon, Lisbon, Portugal
Abstract
ABSTRACT
We screened 615 gram-positive isolates from 150 healthy children for the presence of the
erm
(A),
erm
(B),
erm
(C),
erm
(F), and
mef
(A) genes. The
mef
(A) genes were found in 20 (9%) of the macrolide-resistant isolates, including
Enterococcus
spp.,
Staphylococcus
spp., and
Streptococcus
spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn
1207.1
, a genetic element carrying
mef
(A) recently described in
Streptococcus pneumoniae
. The three
Staphylococcus
spp. did not carry
orf1
to
orf3
. A gram-negative
Acinetobacter junii
isolate also carried the other seven ORFs described in Tn
1207.1.
A
Staphylococcus aureus
isolate, a
Streptococcus intermedius
isolate, a
Streptococcus
sp. isolate, and an
Enterococcus
sp. isolate had their
mef
(A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the
mef
(A) gene from
S. pneumoniae
.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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