Comparison of
Helicobacter pylori
Virulence Gene Expression In Vitro and in the Rhesus Macaque
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Published:2005-08
Issue:8
Volume:73
Page:4895-4904
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ISSN:0019-9567
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Container-title:Infection and Immunity
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language:en
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Short-container-title:Infect Immun
Author:
Boonjakuakul Jenni K.12, Canfield Don R.3, Solnick Jay V.124
Affiliation:
1. Departments of Medical Microbiology and Immunology 2. Internal Medicine 3. California National Primate Research Center 4. Center for Comparative Medicine, University of California at Davis, Davis, California 95616
Abstract
ABSTRACT
We used a quantitative real-time reverse transcriptase PCR assay to measure the transcript abundance of 46 known and putative
Helicobacter pylori
virulence genes, including 24 genes on the Cag pathogenicity island. The expression profile of
H. pylori
cells grown in vitro was also compared to expression in vivo after experimental infection of rhesus macaques. Transcript abundance in vitro (mid-log phase) ranged from about 0.004 (
feoB
and
hpaA
) to 20 (
ureAB
,
napA
, and
cag25
) copies/cell. Expression of most genes was repressed during the transition from logarithmic- to stationary-phase growth, but several well-characterized
H. pylori
virulence genes (
katA
,
napA
,
vacA
, and
cagA
) were induced. Comparison of results in the rhesus macaque with similar data from humans showed a strong correlation (
r
= 0.89). The relative in vivo expression in the rhesus monkey was highly correlated with in vitro expression during mid-log (
r
= 0.89)- and stationary (
r
= 0.88)-phase growth. Transcript abundance was on average three- to fourfold reduced in vivo compared to in vitro during mid-log phase. However, when compared to stationary phase, increased expression in vivo was observed for 6 of 7 genes on a contiguous portion of the pathogenicity island, several of which are thought to encode the
H. pylori
type IV structural pilus and its accessory proteins. These results suggest the possibility that some genes encoding the
H. pylori
type IV structural pilus and accessory proteins may form an operon that is induced during growth in vivo.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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