Genome and Functional Characterization of Colonization Factor Antigen I- and CS6-Encoding Heat-Stable Enterotoxin-Only Enterotoxigenic Escherichia coli Reveals Lineage and Geographic Variation

Author:

Hazen Tracy H.12,Nagaraj Sushma12,Sen Sunil3,Permala-Booth Jasnehta3,Del Canto Felipe4,Vidal Roberto45,Barry Eileen M.23,Bitoun Jacob P.6ORCID,Chen Wilbur H.3,Tennant Sharon M.3,Rasko David A.12ORCID

Affiliation:

1. Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA

2. Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA

3. Center for Vaccine Development, Institute for Global Health and Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA

4. Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile

5. Instituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile

6. Department of Microbiology & Immunology, Tulane University School of Medicine, New Orleans, Louisiana, USA

Abstract

Comparative genomics and functional characterization were used to analyze a global collection of CFA/I and CS6 ST-only ETEC isolates associated with human diarrhea, demonstrating differences in the genomic content of CFA/I and CS6 isolates related to CF type, lineage, and geographic location of isolation and also lineage-related differences in ST production. Complete genome sequencing of selected CFA/I and CS6 isolates enabled descriptions of a highly conserved ST-positive (ST + ) CFA/I plasmid and of at least five diverse ST and/or CS6 plasmids among the CS6 ETEC isolates. There is currently no approved vaccine for ST-only ETEC, or for any ETEC for that matter, and as such, the current report provides functional verification of ST and CF production and antimicrobial susceptibility testing and an in-depth genomic characterization of a collection of isolates that could serve as representatives of CFA/I- or CS6-encoding ST-only ETEC strains for future studies of ETEC pathogenesis, vaccine studies, and/or clinical trials.

Funder

HHS | National Institutes of Health

Fondo Nacional de Desarrollo Científico y Tecnológico

PATH

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modelling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

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