Regulatable Vectors for Environmental Gene Expression in Alphaproteobacteria-Reference-Cited by-同舟云学术

Regulatable Vectors for Environmental Gene Expression in Alphaproteobacteria

Published:2012-10 Issue:19 Volume:78 Page:7137-7140
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Tett Adrian J.¹,Rudder Steven J.¹,Bourdï¿½s Alexandre¹,Karunakaran Ramakrishnan¹,Poole Philip S.¹

Affiliation:

1. Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom

Abstract

ABSTRACT Two expression vectors utilizing the inducible taurine promoter ( tauAp ) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta . The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01188-12

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