Stabilisation of the RirA [4Fe–4S] cluster results in loss of iron-sensing function-Reference-Cited by-同舟云学术

Stabilisation of the RirA [4Fe–4S] cluster results in loss of iron-sensing function

Published:2023 Issue:36 Volume:14 Page:9744-9758
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Gray Elizabeth¹^ORCID,Stewart Melissa Y. Y.¹,Hanwell Libby²,Crack Jason C.¹^ORCID,Devine Rebecca³^ORCID,Stevenson Clare E. M.³^ORCID,Volbeda Anne⁴^ORCID,Johnston Andrew W. B.²,Fontecilla-Camps Juan C.⁴^ORCID,Hutchings Matthew I.³^ORCID,Todd Jonathan D.²^ORCID,Le Brun Nick E.¹^ORCID

Affiliation:

1. Centre for Molecular and Structural Biochemistry, School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK

2. School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK

3. Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK

4. Metalloproteins Unit, Institut de Biologie Structurale, CEA, CNRS, Université Grenoble-Alpes, 71, Avenue des Martyrs, CS 10090, 38044 Grenoble Cedex 9, France

Abstract

The regulator RirA senses Fe through its [4Fe–4S] cluster, which may have only three Cys ligands from the protein. Addition of a likely fourth Asp ligand stabilised the cluster, rendering it unable to sense Fe and locked into its DNA-binding state.

Funder

Agence Nationale de la Recherche

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D3SC03020B

Reference47 articles.

1. Evidence that the Rhizobium regulatory protein RirA binds to cis-acting iron-responsive operators (IROs) at promoters of some Fe-regulated genes

2. RirA, an iron-responsive regulator in the symbiotic bacterium Rhizobium leguminosarum The GenBank accession number for the RirA sequence is CAC35510.